Halolink resin
WebOct 31, 2015 · Detection of HaloTag-fusion protein is achieved through binding to a fluorescent chloroalkane ligand, enabling rapid optimization of expression levels. HaloTag-based purification uses covalent capture of the HaloTag fusion onto HaloLink resin coupled with proteolytic cleavage to release the protein of interest from the resin. WebHaloLink® ResinはHaloTag®融合タンパク質を固相表面に方向性を維持しながら共有結合で捕捉するためのレジンです。 このレジンはSepharose®ビーズにHaloTag®リガンド …
Halolink resin
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WebHaloTEV Protease is a fusion between the HaloTag® protein and TEV protease, a highly specific proteolytic enzyme that cleaves at a specific TEV site, a specific seven-amino-acid sequence (ENLYFQ (G/S)). HaloTEV Protease enables covalent immobilization on HaloLink™ Resin and removal from a cleavage reaction in a single-step purification. WebThe HaloLink Resin can be used in a variety of applications including: detection and analysis of protein:protein interactions (in vivo and in vitro), detection of enzymatic …
WebThe HaloLink Resin provides a method for covalent and oriented attachment of HaloTag™ fusion proteins onto a solid surface. $199.00 - $1203.00 Specifications View More Specs … WebThe HaloLink™ Resin allows covalent and oriented surface immobilization of HaloTag™ fusion proteins. HaloLink™ Resin consists of Sepharose® beads with a HaloTag™ …
WebHaloTag ® Protein Purification System each G6280 Includes: • 100ml HaloLink™ Resin, 100ml of 25% slurry • 5ml HisLink™ Protein Purification Resin, 50% slurry • 1.6ml TEV Protease, for use with HisLink™ Resin TEV Protease Storage Buffer: 50mM HEPES, 1mM DTT, 0.5mM EDTA, 125mM NaCl, 50% Glycerol, 0.1% Triton. WebThe HaloLink™ Resin can be used in a variety of applications including: detection and analysis of protein:protein interactions (in vivo and in vitro), detection of enzymatic …
WebHaloLink™ Resin, improve the rate of successful complex capture and identification from mammalian cells. The HaloTag® Mammalian Pull-Down and Labeling System (Cat.# G6500) also includes the HaloTag® TMR Direct™ Ligand, which allows the study of cellular localization, trafficking and protein turnover using the same HaloTag® genetic construct.
WebJan 1, 2014 · HaloLink™ Resin (Promega). 5. HaloTEV Protease (Promega). 6. Calf Intestinal Alkaline Phosphatase (CIP; NEB). 7. T4 Polynucleotide Kinase (T4 PNK; NEB). 8. γ- 32 P-ATP (10 μCi/μl). 9. T4 RNA Ligase (Fermentas) ( see Note 1 ). 10. 10 mM ATP solution. 11. Protein electrophoresis size markers. 12. 4× NuPAGE ® LDS Sample Buffer … granger high school addressWebPromega HaloTag Protein Purification System Contains 10mL of a 25% slurry Fisher Scientific Fisher Scientific Fisher Healthcare Fisher Science Education Sign Up for Email NEW Help & Support Center ›+1-800-766-7000 0 No offer available Chemical Structure Certificates Safety Data Sheets Order Status Special Offers & Programs granger high school class of 1965WebJan 24, 2024 · Highly sensitive protein quantification enables the detection of a small number of protein molecules that serve as markers/triggers for various biological phenomena, such as cancer. Here, we describe the development of a highly sensitive protein quantification system called HaloTag protein barcoding … granger health clinicWebHaloLink™ Resin provides a method for specific, covalent and oriented immobilization of proteins onto surfaces. Here we demonstrate the high binding capacity and minimal nonspecific binding of the HaloLink™ Resin. We also show that the covalent HaloTag® bond provides stability during dilution and stringent washing, minimizing the loss of ... chin fasciculationsWebHaloTag® is a protein fusion tag engineered to form a highly specific, covalent bond with HaloLink™ Resin. This covalent binding coupled with the low nonspecific binding of the HaloLink™ Resin provide superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels. granger high school catalogWebHaloLink™ Resin provides a method for specific, covalent and oriented immobilization of proteins onto surfaces. Here we demonstrate the high binding capacity and minimal nonspecific binding of the HaloLink™ Resin. chinfants head shaping helmetsWeb丁香通为您提供time试剂盒m07cd商品详情介绍：价格：询价，货号：11017000050，品牌：佰诺全景，产地：中国，详见丁香通time试剂盒m07cd商品详情页； granger high school calendar