Phenol hydroxylase
Web22. apr 2003 · Phenol hydroxylase (PH) from Acinetobacter radioresistens S13 represents an example of multicomponent aromatic ring monooxygenase made up of three moieties: a reductase (PHR), an oxygenase (PHO) and a regulative component (PHI). WebPhenol Catechol hydroxylase 1.2 - oxygenase The ring-cleaving enzyme from this organism, catechol I,Z-oxygenase, has been pursed and studied in detail [29]. The ring-hydroxylating enzyme, phenol hydroxylase, has been obtained in cell-free extract and shown to require NADPH for its activity, which was further stimulated by FAD [28].
Phenol hydroxylase
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WebThe phenol hydroxylase activity was measured using a modified Neujahr’s method (Neujahr and Kjellén, 1980). The 200-μL enzymatic reaction mixture containing 0.2 mM FAD, 0.1 mM NADPH, 1 mM phenol, and 0.1 M K 3 PO 4 buffer (pH 7.5), and 50 μL crude enzyme was incubated at 30°C and the absorbance (A) at 340 nm was read every 30 s. The above ... Web25. dec 2001 · phenol hydroxylase PHR PH reductase Phenol is a widely distributed environmental pollutant generating a strong interest in strategies for bioremediation [ 1 ]. In the last 20 years, studies have been performed on both aerobic [ 2] and anaerobic [ 3] treatment of aromatic pollutants by using pure microorganisms or mixed cultures.
Web2. jún 2024 · The hydroxylation of phenols into polyphenols, which are valuable chemicals and pharmaceutical products, is a challenging reaction. The search for green synthetic processes has led to considering microorganisms and pure hydroxylases as catalysts for phenol hydroxylation.
Web2. dec 2006 · Phenol hydroxylase (PH) belongs to a family of bacterial multicomponent monooxygenases (BMMs) with carboxylate-bridged diiron active sites. Included are toluene/o-xylene (ToMO) and soluble methane (sMMO) monooxygenase. PH hydroxylates aromatic compounds, but unlike sMMO, it cannot oxidize alkanes despite having a similar … Web15. máj 1998 · Thus, phenol hydroxylase is a member of a family of flavin-containing aromatic hydroxylases that share the same overall fold, in spite of large differences in …
Web28. nov 2003 · PheA1 catalyzes the efficient ortho-hydroxylation of phenol to catechol when supplemented with PheA2 and FAD/NADH. The hydroxylase activity is strictly FAD …
Web28. nov 2003 · Phenol hydroxylase activity was measured at 50 °C. The reaction mixture contained 0.1 m m phenol, 1 m m NADH, and 10 μ m FAD in 50 m m potassium phosphate (pH 7.0). a In the absence of 1 m m PheA2. b In the presence of 1 m m PheA2. Download : Download full-size image Fig. 2. christian robles washington stateWeb7. nov 2012 · The enzyme catalyzing these reactions and having a key role in the aerobic degradation of monoaromatic compounds is a monooxygenase: phenol hydroxylase (EC 1.14.13.7) 78. Aromatic monooxygenases are divided into two groups: monocomponent and multicomponent enzyme forms 79. christian roberts actor moviesWeb13. sep 2012 · For phenol hydroxylase activity, assay mixtures contained 50 mM Tris-HCl (pH 8.0), 2.5 µM NADH, 0.02 mg protein and 200 mg/L phenol. Each assay was started … christian robinson ufWeb1. apr 1998 · Abstract. The new thermophilic Bacillus thermoleovorans strain A2 degrades phenol and cresols via the meta cleavage pathway. The first two enzymes involved in this process, the phenol hydroxylase and catechol 2,3-dioxygenase, encoded by the pheA and pheB genes respectively, were cloned and sequenced.The deduced amino acid sequence … christian robinson spokane waWebPhenol and its derivatives (alkylphenols, halogenated phenols, nitrophenols) are natural or man-made aromatic compounds that are ubiquitous in nature and in human-polluted … christian robinson illustrator youtubeWeb1. jan 2024 · Phenolic hydroxylases belong to Groups A and D of flavin-dependent monooxygenases [25], [29]. Monooxygenases are defined as enzymes that can incorporate a single atom of molecular oxygen into an organic substrate. The monooxygenation of phenolic compounds can typically be carried out by the hydroxylation or formation of … christian robles mdWebThe consortium exhibited activity of phenol hydroxylase and catechol 1,2-dioxygenase suggesting that members in consortium followed ortho pathway for phenol degradation. Response surface methodology (RSM) has been adopted to evaluate the effect of different parameter such as initial phenol concentration, temperature, pH and time on phenol ... christian robinson you matter read aloud